Composite

Part:BBa_K1632030:Design

Designed by: Riku Shinohara   Group: iGEM15_Tokyo_Tech   (2015-08-30)


fim switch[default ON](Tokyo_Tech/J23119)_rbs_gfp_rbs_rhlI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 345
    Illegal NheI site found at 368
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 374
    Illegal BglII site found at 1865
    Illegal BamHI site found at 333
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1102


Design Notes

sequence confirmed

Materials and Methods

Source

Composite of BBa_K1632002, BBa_I9026

References